Effect of graphene oxide on affinity-immobilization of purple membranes on solid supports.
Identifieur interne : 000207 ( Main/Exploration ); précédent : 000206; suivant : 000208Effect of graphene oxide on affinity-immobilization of purple membranes on solid supports.
Auteurs : RBID : pubmed:24561502Abstract
The effect of graphene oxide (GO) on the surface fabrication of purple membranes (PM) containing photosensitive bacteriorhodopsin is first reported in this study. GO was initially modified with biotin and then coupled with oxidized avidin to generate a GO-avidin complex, which was subsequently used as a linker to immobilize biotinylated PM (b-PM) onto amine-functionalized supports. Indium-tin-oxide glass coated with the GO-avidin complex was more hydrophilic than the electrode coated only with oxidized avidin, and the successive b-PM adsorption yielded a 1.4-fold higher (410nA/cm(2)) photoelectric activity. AFM analysis on mica revealed that the GO-avidin complex layer had less surface roughness and dissipation energy than the pure oxidized avidin linker layer. For subsequent b-PM fabrication, GO addition not only reduced the stacking of immobilized b-PM patches but also improved their interior compactness and surface smoothness. This study demonstrates a convenient way to introduce GO into PM fabrication technology to provide enhanced surface morphology and photoelectric activity.
DOI: 10.1016/j.colsurfb.2014.01.035
PubMed: 24561502
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Effect of graphene oxide on affinity-immobilization of purple membranes on solid supports.</title><author><name sortKey="Chen, Hsiu Mei" uniqKey="Chen H">Hsiu-Mei Chen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10617, Taiwan. Electronic address: hsiumei@mail.ntust.edu.tw.</nlm:affiliation><country xml:lang="fr">République de Chine (Taïwan)</country><wicri:regionArea>Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10617</wicri:regionArea></affiliation></author><author><name sortKey="Lin, Chi Jung" uniqKey="Lin C">Chi-Jung Lin</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10617, Taiwan.</nlm:affiliation><country xml:lang="fr">République de Chine (Taïwan)</country><wicri:regionArea>Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10617</wicri:regionArea></affiliation></author><author><name sortKey="Jheng, Kai Ru" uniqKey="Jheng K">Kai-Ru Jheng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10617, Taiwan.</nlm:affiliation><country xml:lang="fr">République de Chine (Taïwan)</country><wicri:regionArea>Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10617</wicri:regionArea></affiliation></author><author><name sortKey="Kosasih, Aline" uniqKey="Kosasih A">Aline Kosasih</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10617, Taiwan.</nlm:affiliation><country xml:lang="fr">République de Chine (Taïwan)</country><wicri:regionArea>Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10617</wicri:regionArea></affiliation></author><author><name sortKey="Chang, Jia Yaw" uniqKey="Chang J">Jia-Yaw Chang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10617, Taiwan.</nlm:affiliation><country xml:lang="fr">République de Chine (Taïwan)</country><wicri:regionArea>Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10617</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><date when="2014">2014</date><idno type="RBID">pubmed:24561502</idno><idno type="pmid">24561502</idno><idno type="doi">10.1016/j.colsurfb.2014.01.035</idno><idno type="wicri:Area/Main/Corpus">000145</idno><idno type="wicri:Area/Main/Curation">000145</idno><idno type="wicri:Area/Main/Exploration">000207</idno></publicationStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The effect of graphene oxide (GO) on the surface fabrication of purple membranes (PM) containing photosensitive bacteriorhodopsin is first reported in this study. GO was initially modified with biotin and then coupled with oxidized avidin to generate a GO-avidin complex, which was subsequently used as a linker to immobilize biotinylated PM (b-PM) onto amine-functionalized supports. Indium-tin-oxide glass coated with the GO-avidin complex was more hydrophilic than the electrode coated only with oxidized avidin, and the successive b-PM adsorption yielded a 1.4-fold higher (410nA/cm(2)) photoelectric activity. AFM analysis on mica revealed that the GO-avidin complex layer had less surface roughness and dissipation energy than the pure oxidized avidin linker layer. For subsequent b-PM fabrication, GO addition not only reduced the stacking of immobilized b-PM patches but also improved their interior compactness and surface smoothness. This study demonstrates a convenient way to introduce GO into PM fabrication technology to provide enhanced surface morphology and photoelectric activity.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="In-Data-Review"><PMID Version="1">24561502</PMID><DateCreated><Year>2014</Year><Month>04</Month><Day>21</Day></DateCreated><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-4367</ISSN><JournalIssue CitedMedium="Internet"><Volume>116</Volume><PubDate><Year>2014</Year><Month>Apr</Month><Day>1</Day></PubDate></JournalIssue><Title>Colloids and surfaces. B, Biointerfaces</Title><ISOAbbreviation>Colloids Surf B Biointerfaces</ISOAbbreviation></Journal><ArticleTitle>Effect of graphene oxide on affinity-immobilization of purple membranes on solid supports.</ArticleTitle><Pagination><MedlinePgn>482-8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.colsurfb.2014.01.035</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0927-7765(14)00036-8</ELocationID><Abstract><AbstractText>The effect of graphene oxide (GO) on the surface fabrication of purple membranes (PM) containing photosensitive bacteriorhodopsin is first reported in this study. GO was initially modified with biotin and then coupled with oxidized avidin to generate a GO-avidin complex, which was subsequently used as a linker to immobilize biotinylated PM (b-PM) onto amine-functionalized supports. Indium-tin-oxide glass coated with the GO-avidin complex was more hydrophilic than the electrode coated only with oxidized avidin, and the successive b-PM adsorption yielded a 1.4-fold higher (410nA/cm(2)) photoelectric activity. AFM analysis on mica revealed that the GO-avidin complex layer had less surface roughness and dissipation energy than the pure oxidized avidin linker layer. For subsequent b-PM fabrication, GO addition not only reduced the stacking of immobilized b-PM patches but also improved their interior compactness and surface smoothness. This study demonstrates a convenient way to introduce GO into PM fabrication technology to provide enhanced surface morphology and photoelectric activity.</AbstractText><CopyrightInformation>Copyright © 2014 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Hsiu-Mei</ForeName><Initials>HM</Initials><Affiliation>Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10617, Taiwan. Electronic address: hsiumei@mail.ntust.edu.tw.</Affiliation></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Chi-Jung</ForeName><Initials>CJ</Initials><Affiliation>Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10617, Taiwan.</Affiliation></Author><Author ValidYN="Y"><LastName>Jheng</LastName><ForeName>Kai-Ru</ForeName><Initials>KR</Initials><Affiliation>Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10617, Taiwan.</Affiliation></Author><Author ValidYN="Y"><LastName>Kosasih</LastName><ForeName>Aline</ForeName><Initials>A</Initials><Affiliation>Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10617, Taiwan.</Affiliation></Author><Author ValidYN="Y"><LastName>Chang</LastName><ForeName>Jia-Yaw</ForeName><Initials>JY</Initials><Affiliation>Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10617, Taiwan.</Affiliation></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>01</Month><Day>31</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Colloids Surf B Biointerfaces</MedlineTA><NlmUniqueID>9315133</NlmUniqueID><ISSNLinking>0927-7765</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Affinity immobilization</Keyword><Keyword MajorTopicYN="N">Bacteriorhodopsin</Keyword><Keyword MajorTopicYN="N">Graphene oxide</Keyword><Keyword MajorTopicYN="N">Purple membrane</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>9</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>1</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>1</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2014</Year><Month>1</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>2</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>2</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>2</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24561502</ArticleId><ArticleId IdType="pii">S0927-7765(14)00036-8</ArticleId><ArticleId IdType="doi">10.1016/j.colsurfb.2014.01.035</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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